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技術文章

Technical articles

當前位置:首頁技術文章Worthington-Biochem代理商及品牌介紹( Worthington特約代理)-上海起發(fā)

Worthington-Biochem代理商及品牌介紹( Worthington特約代理)-上海起發(fā)

更新時間:2026-03-23點擊次數(shù):22

一、公司介紹

自1947年創(chuàng)立以來,Worthington-Biochem始終致力于為生命科學領域提供高純度酶制劑及相關生化試劑。作為酶學領域的優(yōu)秀者,公司以“推動科研突破與生物工藝革新"為核心使命,憑借深厚的技術積淀和持續(xù)的創(chuàng)新能力,成為100多個國家科研機構、生物制藥企業(yè)及診斷實驗室的合作伙伴。  

從經(jīng)典的細胞分離酶到前沿的無動物源產(chǎn)品線,從分子生物學級核酸酶到定制化生物工藝解決方案,Worthington-Biochem的產(chǎn)品覆蓋生命科學研究的全鏈條。其技術優(yōu)勢源于對酶活性、穩(wěn)定性和安全性的追求,以及對客戶需求快速響應的專業(yè)服務團隊。  


?? 下圖為"上海起發(fā)"作為其授權代理的授權書

Worthington-biochem.jpg

二、核心優(yōu)勢

1. 78年技術積淀,專注酶學領域  

   • 深耕酶純化技術,從動物組織、植物提取到微生物發(fā)酵,掌握酶來源篩選與活性優(yōu)化方案。  

   • 產(chǎn)品線覆蓋7大類、數(shù)百種酶制劑,滿足從基礎研究到大規(guī)模生產(chǎn)的多樣化需求。  

2. 科學驗證,實驗可重復性保障  

   • 每批次產(chǎn)品提供詳細的生化參數(shù)(如比活、蛋白含量、內(nèi)毒素水平),確保實驗結果穩(wěn)定可靠。  

   • 與哈佛醫(yī)學院、中科院等機構合作,累計支持超5600篇科研文獻發(fā)表。  

3. 嚴格的質量管理體系  

   • 生產(chǎn)流程,從原料采購到成品包裝全程可追溯。  

   • 無動物源(Animal Free)系列產(chǎn)品通過GMP兼容性驗證,消除BSE/TSE及哺乳動物病毒風險。  

4. 定制化服務能力  

   • 針對生物制藥、疫苗生產(chǎn)等場景,提供酶活性優(yōu)化、配方調整等專業(yè)支持。  

   • 技術團隊提供實驗方案設計、數(shù)據(jù)分析等全流程服務。  


三、熱門產(chǎn)品介紹

1. 膠原蛋白酶(Collagenase)

• 產(chǎn)品特點:  

  • 提供7種亞型(Type I-IV、Purified等),適配脂肪、肝臟、心臟、腫瘤等不同組織解離需求。  

  • 無動物源配方(STEMxyme®系列)支持干細胞研究與臨床轉化。  

• 存儲條件:-20℃避光保存,避免反復凍融。  

• 工作原理:特異性降解膠原蛋白中的Pro-Y-Gly-Pro序列,協(xié)同中性蛋白酶、透明質酸酶分解細胞間基質。  

• 使用方法:根據(jù)組織類型調整酶濃度(通常1-2 mg/mL)與孵育時間(30分鐘至2小時),配合機械剪切優(yōu)化解離效率。  

2. 脫氧核糖核酸酶I(DNase I)

• 產(chǎn)品特點:  

  • 分為研究級(DPRF/DPRFS)與重組級(DR1/DR1S),RNase和蛋白酶污染低于檢測限。  

  • 適用于RNA提取、RT-PCR前處理及生物制藥工藝中的DNA清除。  

• 存儲條件:溶液型-20℃,凍干粉2-8℃。  

• 工作原理:非特異性切割DNA的3',5'-磷酸二酯鍵,優(yōu)先消化基因組DNA。  

• 使用方法:在RNA樣品中加入1-2 U/μL DNase I,37℃孵育30分鐘,通過酚氯仿抽提去除酶殘留。  

3. 彈性蛋白酶(Elastase)

• 產(chǎn)品特點:  

  • 純化自豬胰腺,高效水解彈性蛋白,適用于肺細胞、腫瘤微環(huán)境研究。  

  • 低蛋白酶活性設計,減少對細胞膜蛋白的損傷。  

• 存儲條件:凍干粉2-8℃,復溶后-20℃短期保存。  

• 工作原理:特異性切割彈性蛋白的Ala-Ala鍵,保留膠原蛋白結構。  

• 使用方法:與膠原酶聯(lián)用(比例1:5),37℃消化肺組織塊1-3小時。  

4. 木瓜蛋白酶(Papain)

• 產(chǎn)品特點:  

  • 從番木瓜乳膠中提取,溫和解離神經(jīng)組織與胚胎樣本。  

  • 支持流式細胞術分選前的細胞預處理。  

• 存儲條件:凍干粉2-8℃,復溶后分裝凍存。  

• 工作原理:水解細胞外基質中的肽鍵,保留細胞表面抗原完整性。  

• 使用方法:10-20 mg/mL濃度,37℃孵育1-2小時,加入半胱氨酸激活酶活。  

5. 胰蛋白酶(Trypsin)

• 產(chǎn)品特點:  

  • TPCK處理型減少自溶,適用于蛋白質組學與測序樣本制備。  

  • 無外泌體殘留配方,保障單細胞測序數(shù)據(jù)準確性。  

• 存儲條件:凍干粉2-8℃,溶液型-20℃。  

• 工作原理:切割賴氨酸與精氨酸的羧基端,釋放單細胞懸液。  

• 使用方法:0.25%濃度(含0.02% EDTA),37℃消化10-30分鐘,胰酶抑制劑終止反應。  


四、解決實驗中的核心問題

1. 細胞分離效率低  

   • 痛點:傳統(tǒng)酶制劑活性不穩(wěn)定,導致組織解離不全。  

   • 解決方案:多酶協(xié)同配方(如膠原酶+中性蛋白酶)精準匹配組織類型,提升細胞得率。  

2. DNA污染影響分子實驗  

   • 痛點:質粒構建或RNA提取中基因組DNA殘留。  

   • 解決方案:DNase I(DPRF級)在無RNase環(huán)境下清除DNA,保障實驗純度。  

3. 動物源成分限制臨床轉化  

   • 痛點:BSE/TSE風險阻礙干細胞治療與疫苗申報。  

   • 解決方案:無動物源膠原酶(CLSAFA)、AF Trypsin等產(chǎn)品符合GMP要求。  

4. 批次間差異干擾結果  

   • 痛點:不同批次酶活性波動導致實驗可重復性差。  

   • 解決方案:每批次提供活性證書(COA),支持客戶定制質檢方案。  


五、目標客戶群體

• 科研機構:高校、研究所的細胞生物學、分子生物學實驗室。  

• 生物制藥企業(yè):疫苗生產(chǎn)、抗體藥物開發(fā)、基因治療研發(fā)部門。  

• 診斷實驗室:需要高特異性酶聯(lián)免疫檢測(ELISA)的技術平臺。  

• 教學機構:醫(yī)學院、生物工程專業(yè)的實驗教學與技能培訓。  


六、購買常見問題與解答

Q1:酶制品的活性單位如何定義?  

A:活性單位基于特定底物反應體系(如膠原酶降解牛跟腱膠原的速率),確保跨批次可比性。  

Q2:無動物源產(chǎn)品是否適用于大規(guī)模生產(chǎn)?  

A:是的,曲霉發(fā)酵來源的DNase I、植物源蛋白酶等已通過中試驗證,支持GMP批次生產(chǎn)。  

Q3:如何選擇適合的膠原酶亞型?  

A:參考組織類型——肝臟優(yōu)選Type IV(低胰酶活性),腫瘤組織推薦Type I+II組合。  

Q4:產(chǎn)品運輸是否全程冷鏈?  

A:干粉產(chǎn)品常溫運輸,液體酶采用干冰冷鏈,確保活性穩(wěn)定。  

Q5:能否提供小樣試用?  

A:支持免費樣品申請,需提供實驗方案以便推薦適配產(chǎn)品。  


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更多產(chǎn)品信息,請聯(lián)系中國區(qū)域代理商:上海起發(fā)實驗試劑有限公司

如果您對任意產(chǎn)品或其他品牌感興趣,可通過“在線留言"功能留下您的需求

(注:本文內(nèi)容基于品牌公開資料及行業(yè)常規(guī)信息整理,具體以品牌信息文檔為準。)


??賣產(chǎn)品

貨號

品名

規(guī)格

品牌

LS004176

Collagenase, Type 2 (CLS-2)2型膠原酶

1gm

Worthington Biochemical

LS004196

Collagenase Type 1

1gm

Worthington Biochemical

LS004188

Collagenase, Type 4

1gm

Worthington Biochemical

LS004194

Collagenase Type 1 (CLS-1)

100mg

Worthington Biochemical

LS004174

Collagenase, Type 2

100mg

Worthington Biochemical

LS004186

Collagenase, Type 4

100mg

Worthington Biochemical

LS004182

Collagese, Type 3

1gm

Worthington Biochemical

ls003126

Papain, Suspension

100mg

Worthington Biochemical

LK003150

Papain Dissociation System

1bx

Worthington Biochemical

LS003119

Papain, Lyophilized

100mg

Worthington Biochemical

LS004180

Collagenase, Type 3

100mg

Worthington Biochemical

LS003740

Trypsin, TPCK Treated

100mg

Worthington Biochemical

LS002139

Deoxyribonuclease I

100mg

Worthington Biochemical

LK003178

PDS Kit Papain Vial

5vi

Worthington Biochemical

LS002292

Elastase Lyophilized

100mg

Worthington Biochemical

LS002279

Elastase, Suspension (ES)

100mg

Worthington Biochemical

LS004177

Collagese, Type 2

5gm

Worthington Biochemical

LS004189

Collagenase, Type 4

5 gm

Worthington Biochemical

LS002006

Deoxyribonuclease I

20mg

Worthington Biochemical

LS003926

Phosphodiesterase I

100un

Worthington Biochemical

LK003176

PDS Kit Papain Vial (PAP2)

1 vi

Worthington Biochemical

LS002007

Deoxyribonuclease I

100mg

Worthington Biochemical

LS05006

Reverse Transcriptase, Recombinant, HIV

5x200 un

Worthington Biochemical

LS002140

Deoxyribonuclease I

1 gm

Worthington Biochemical

LK003170

DNase Vial (D2) (D2

1 vi

Worthington Biochemical

LK003182

PDS Kit Inhibitor Vial (OI-BSA)

1 vi

Worthington Biochemical

LS002594

Hyaluronidase

50ku

Worthington Biochemical

LS002290

Elastase, Lyophilized

25mg

Worthington Biochemical

LS004106

STEMxyme® 1, Collagenase/Neutral Protease (Dispase®), Animal Free

50mg

Worthington Biochemical

LS02100

Neutral Protease (Dispase), Purified

10mg

Worthington Biochemical

LS003570

Trypsin Inhibitor, Soybean, Purified

100mg

Worthington Biochemical

LS003608

Protease, S. Aureus (Endoproteinase Glu-C)

1mg

Worthington Biochemical

LS002274

Elastase, Suspension

25mg

Worthington Biochemical

LS003127

Papain, Suspension

1 gm

Worthington Biochemical

LS003086

Trypsin Inhbitor, Ovomucoid

2 gm

Worthington Biochemical

LS005305

Carboxypeptidase B

5mg

Worthington Biochemical

LS004212

Collagenase, Type 4, Filtered Store at 2-8°C.

5x50mg

Worthington Biochemical

LS003118

Papain, Lyophilized

25mg

Worthington Biochemical

LS004200

Collagenase, Type 1

Bulk

Worthington Biochemical

LS004179

Collagese, Type 2

Bulk

Worthington Biochemical

LS004197

Collagenase Type 1

5 gm

Worthington Biochemical

LS004798

Nuclease, Micrococcal

45 ku

Worthington Biochemical

LS003120

Papain, Lyophilized

1 gm

Worthington Biochemical

LS004191

Collagenase, Type 4

500g

Worthington Biochemical

LS006344

Deoxyribonuclease I, RNase & Protease Free, Solution

500 un

Worthington Biochemical

LS001070

Alcohol Dehydrogese, Lyophilized

1gm

Worthington Biochemical

LS002592

Hyaluronidase

300ku

Worthington Biochemical

LS02104

Neutral Protease (Dispase), Purified

50mg

Worthington Biochemical

LS003124

Papain, Suspension

25mg

Worthington Biochemical

LS003571

Trypsin Inhibitor, Soybean, Purified

1 gm

Worthington Biochemical

LS004520

Galactose Oxidase

150 un

Worthington Biochemical

LS004522

Galactose Oxidase

450 un

Worthington Biochemical

LS002138

Deoxyribonuclease I

25mg

Worthington Biochemical

LS003793

Polyphenol Oxidase (Tyrosinase)

500 ku

Worthington Biochemical

LS004524

Galactose Oxidase

1 ku

Worthington Biochemical

LK003163

Papain Dissociation System, Without EBSS

3 bx

Worthington Biochemical

LS003605

Protease, S. Aureus (Endoproteinase Glu-C)

5mg

Worthington Biochemical

LS003744

Trypsin, TPCK Treated

1 gm

Worthington Biochemical

LS005275

Collagenase, Purified

4ku

Worthington Biochemical

LS006323(500ku)

Deoxyribonuclease I, Recombint, Animal Free, Bioprocess Grade

500ku

Worthington Biochemical

LS02111

Neutral Protease, Partially Purified

5gm

Worthington Biochemical

LS003602

Phosphodiesterase II

25un

Worthington Biochemical

LS006363

Elastase, Purified

5mg

Worthington Biochemical

LS005282

Collagenase, Type 5

1gm

Worthington Biochemical

LS02122

Trypsin, Modified, SequENZ® Sequencing Grade

4x100ug

Worthington Biochemical

LS003741

Trypsin, TPCK Treated (TRTPCK)

500mg

Worthington Biochemical

LS05003

Reverse Transcriptase, Recombinant, HIV

200 un

Worthington Biochemical

LS006123

Phosphatase, Alkaline, Purified

25mg

Worthington Biochemical

LS002933

Lysozyme, Purified, Salt Free

5 gm

Worthington Biochemical

LS003587

Trypsin Inhibitor, Soybean

1 gm

Worthington Biochemical

LS01492

Ribonuclease T1, Chromatographically Purif., Lyophilized

2500ku

Worthington Biochemical

LK003172

DNase Vial (D2)

5 vi

Worthington Biochemical

LS002106

Deoxyribonucleic Acid, Calf Thymus

1 gm

Worthington Biochemical

LS003887

Urease

10 gm

Worthington Biochemical

LK003153

Papain Dissociation System

3bx

Worthington Biochemical

LS001263

Carbonic Anhydrase

250mg

Worthington Biochemical

LS006323

Deoxyribonuclease I, Recombinant, Animal Free, Bioprocess Grade

500ku

Worthington Biochemical

LS001656

Collagen

10 gm

Worthington Biochemical

LS001334

Chymotrypsin, Alpha, 1X

10 gm

Worthington Biochemical

LS005477

Hyaluronidase, Purified

5ku

Worthington Biochemical

LS004081

Phosphatase, Alkaline

10mg

Worthington Biochemical

LS004780

Neuraminidase

10mg

Worthington Biochemical

LS003589

Trypsin Inhibitor, Soybean

10gm

Worthington Biochemical

LS004154

Collagenase, Type A, Animal Free

1gm

Worthington Biochemical

LS001141

Phosphatase, Acid

1gm

Worthington Biochemical

LS004185

Collagenase, Type 3

500g

Worthington Biochemical

LK003300

Neonatal Cardiomyocyte Isolation System

1 kt

Worthington Biochemical

LS003750

Trypsin, TPCK-Treated, Irradiated

100mg

Worthington Biochemical

LS006331

Deoxyribonuclease I, Ribonuclease & Protease Free

2500un

Worthington Biochemical

LK003188

PDS Kit, EBSS Via

1vi

Worthington Biochemical

LS003981

Glucose-6-Phosphate Dehydrogenase, Lyophilized

1 ku

Worthington Biochemical

LS005273

Collagenase, Purified

10 ku

Worthington Biochemical

LS003707

Trypsin, Purified

1 gm

Worthington Biochemical

LS01502

Ribonuclease T2, Recombinant

250ku

Worthington Biochemical

LS006355

Deoxyribonuclease I, Recombinant, Solution

5x2 ku

Worthington Biochemical

LS02123

Trypsin, Modified, Sequencing Grade

1mg

Worthington Biochemical

LS004210

Collagenase, Type 4, Filtered

50mg

Worthington Biochemical

LS002512

Hexokinase, Lyophilized

10 ku

Worthington Biochemical

LS006308

Amino Acid Oxidase, D-

25mg

Worthington Biochemical

LS004326-50ku

Diaphorase

50ku

Worthington Biochemical